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Title   두경부편평상피암 세포주의 Spheroid 및 Raft 배양에서 IFN - γ 의 효과 ( Effects of IFN - γ on Spheroid and Raft Culture of Squamous Cell Carcinoma of the Head and Neck )
Publicationinfo   1998 Jan; 030(03): 573-583.
Key_word   Multicellular tumor spheroid, Raft culture, Interferon-￥a, Squamous cell carcinoma of the head and neck
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Abstract   Purpose: To establish new in vitro model systems that better reflect in vivo condition, multicellular tumor spheroids(MTS) and raft culture were developed using cell lines of squamous cell carcinoma(SCCHN) of the head and neck. In these 3-dimensional systems, the expression of cell surface molecules which are important for modulation of physiology of tumor cells were studied with or without the treatment of interferon(IFN)-￥a. Materials and Methods: Four SCCHN cell lines were used for MTS and raft culture. The effects of interferon-￥a on SCCHN cells were examined by immunohistochemistry. Results: All cell lines formed MTS, but only Tu-138 showed a good stratification at the air-liquid interface in the raft culture system. Immunohistochemical studies of MTS using monoclonal antibodies revealed a strong staining for MHC class I, no staining for MHC-DR, a weak patch expression of ICAM-1 and a central strong staining for integrin a 6. Staining patterns were similar for the raft cultures except integrin a 6(intense full-thickness positivity). In both systems, IFN-￥a enhanced the expression of MHC-DR and ICAM-1. No significant change was found in the expression of MHC class I and integrin a 6. Conclusions: MTS and raft culture system were established successfully from the SCCHN cell lines. IFN-￥a can modulate the surface molecules of tumor cells in the 3-dimensional culture systems.
저 자   이승주(Seung Ju Lee),김춘동(Chun Dong Kim),고태용(Tae Young Koh),장근호(Keun Ho Chang),이재서(Chae Seo Rhee),윤성준(Seong Jun Yoon),이상구(Sagn Goo Lee),이현주(Hyun Ju Lee),김광현(Kwang Hyun Kim),성명훈(Kwang Hyun Kim)